5.5D11 - Recognises the capsid protein of Chikungunya virus – suitable for use in IFA, ELISA and Western blot.

This purchase is for an approximately 20 microgram/ml aliquot of supernatant that has not been further purified.  Costs for standard shipping are included in the price.

The product data sheet can be downloaded here.   For technical enquiries, please email mozzymabs@uq.edu.au

The price exclude shipping.  Shipping will be organised and charged to your shipping agent account.  We can only ship with FedEx outside of Australia; we can only ship with LabCabs, TNT or Toll within Australia.  Please email your shipping agent account number to mozzymabs@uq.edu.au or we will not be able to process your order.

Target: Chikungunya virus (CHIKV) capsid protein

Produced in: Mouse

Isotype: IgG1

Specificity: Recognises CHIKV strains in the two major genotypes (Asian & East/Central/South African). Also recognises the alphaviruses Ross River virus, Semliki Forest virus and Sindbis virus in ELISA.

Applications: For Research Use Only.  IFA, ELISA and Western blot.

IFA: shown to be effective following fixation using 4% formaldehyde or 100% acetone.

ELISA: Can be used in fixed cell ELISA following 4% formaldehyde or ≥ 20% acetone.

Storage: Contains 0.02% sodium azide as a preservative. Store at 4 °C.

Can be aliquoted and stored frozen, but repeated freeze/thawing should be avoided.

A safety data sheet for 0.05% sodium azide solution can be accessed here.

Publications

Goh LY, Hobson-Peters J, Prow NA, Gardner J, Bielefeldt-Ohmann H, Suhrbier A, Hall RA. (2015) Monoclonal antibodies specific for the capsid protein of chikungunya virus suitable for multiple applications. J Gen Virol. 96:507-12. 

Goh LY, Hobson-Peters J, Prow NA, Baker K, Piyasena TB, Taylor CT, Rana A, Hastie ML, Gorman JJ, Hall RA. (2015) The Chikungunya Virus Capsid Protein Contains Linear B Cell Epitopes in the N- and C-Terminal Regions that are Dependent on an Intact C-Terminus for Antibody Recognition. Viruses. 8;7:2943-64.

THIS MATERIAL IS FOR RESEARCH USE ONLY AND CANNOT BE USED FOR CLINICAL OR DIAGNOSTIC PURPOSES

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