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5.2H8 – Recognises the E2 protein of Chikungunya virus – suitable for use in IFA, ELISA, Western blot and antigen capture
This purchase is for an approximately 20 microgram/ml aliquot of supernatant that has not been further purified.
The product data sheet can be downloaded here.
Target: Chikungunya virus (CHIKV) E2 protein
Produced in: Mouse
Specificity: Recognises CHIKV strains in the two major genotypes (Asian & East/Central/South African). Does not recognise the alphaviruses Ross River virus, Semliki Forest virus, Sindbis virus or Barmah Forest virus in ELISA.
Applications: For Research Use Only. IFA, ELISA, Western blot and antigen capture ELISA.
IFA: shown to be effective following fixation using 4% formaldehyde or 100% acetone.
ELISA: Can be used in fixed cell ELISA following 4% formaldehyde or ≥ 20% acetone.
Storage: Contains 0.02% sodium azide as a preservative. Store at 4 °C.
Can be aliquoted and stored frozen, but repeated freeze/thawing should be avoided.
A safety data sheet for 0.05% sodium azide solution can be accessed here.
Goh LY, Hobson-Peters J, Prow NA, Gardner J, Bielefeldt-Ohmann H, Pyke AT, Suhrbier A, Hall RA. (2013) Neutralizing monoclonal antibodies to the E2 protein of chikungunya virus protects against disease in a mouse model. Clin Immunol. 149(3):487-97.
For technical enquiries, please email email@example.com
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