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4.1H1 - Recognises the capsid protein of Chikungunya virus – suitable for use in IFA, ELISA and Western blot.
This purchase is for an approximately 20 microgram/ml aliquot of supernatant that has not been further purified. Costs for standard shipping are included in the price.
The price exclude shipping. Shipping will be organised and charged to your shipping agent account. We can only ship with FedEx outside of Australia; we can only ship with LabCabs, TNT or Toll within Australia. Please email your shipping agent account number to [email protected] or we will not be able to process your order.
Target: Chikungunya virus (CHIKV) capsid protein
Produced in: Mouse
Specificity: Recognises CHIKV strains in the two major genotypes (Asian & East/Central/South African). Low affinity binding to the alphaviruses Ross River virus and Semliki Forest virus in ELISA.
Applications: For Research Use Only. IFA, ELISA, Western blot.
IFA: shown to be effective following fixation using 4% formaldehyde or 100% acetone.
ELISA: Can be used in fixed cell ELISA following 4% formaldehyde or ≥ 20% acetone.
Storage: Contains 0.02% sodium azide as a preservative. Store at 4 °C.
Can be aliquoted and stored frozen, but repeated freeze/thawing should be avoided.
A safety data sheet for 0.05% sodium azide solution can be accessed here.
Goh LY, Hobson-Peters J, Prow NA, Gardner J, Bielefeldt-Ohmann H, Suhrbier A, Hall RA. (2015) Monoclonal antibodies specific for the capsid protein of chikungunya virus suitable for multiple applications. J Gen Virol. 96:507-12.
Goh LY, Hobson-Peters J, Prow NA, Baker K, Piyasena TB, Taylor CT, Rana A, Hastie ML, Gorman JJ, Hall RA. (2015) The Chikungunya Virus Capsid Protein Contains Linear B Cell Epitopes in the N- and C-Terminal Regions that are Dependent on an Intact C-Terminus for Antibody Recognition. Viruses. 8;7:2943-64.
Taylor A, Liu X, Zaid A, Goh LY, Hobson-Peters J, Hall RA, Merits A, Mahalingam S. (2017) Mutation of the N-Terminal Region of Chikungunya Virus Capsid Protein: Implications for Vaccine Design. mBio. 21;8(1).
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