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4.1H1 - Recognises the capsid protein of Chikungunya virus – suitable for use in IFA, ELISA and Western blot.
This purchase is for an approximately 20 microgram/ml aliquot of supernatant that has not been further purified. Costs for standard shipping are included in the price.
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Target: Chikungunya virus (CHIKV) capsid protein
Produced in: Mouse
Specificity: Recognises CHIKV strains in the two major genotypes (Asian & East/Central/South African). Low affinity binding to the alphaviruses Ross River virus and Semliki Forest virus in ELISA.
Applications: For Research Use Only. IFA, ELISA, Western blot.
IFA: shown to be effective following fixation using 4% formaldehyde or 100% acetone.
ELISA: Can be used in fixed cell ELISA following 4% formaldehyde or ≥ 20% acetone.
Storage: Contains 0.02% sodium azide as a preservative. Store at 4 °C.
Can be aliquoted and stored frozen, but repeated freeze/thawing should be avoided.
A safety data sheet for 0.05% sodium azide solution can be accessed here.
Goh LY, Hobson-Peters J, Prow NA, Gardner J, Bielefeldt-Ohmann H, Suhrbier A, Hall RA. (2015) Monoclonal antibodies specific for the capsid protein of chikungunya virus suitable for multiple applications. J Gen Virol. 96:507-12.
Goh LY, Hobson-Peters J, Prow NA, Baker K, Piyasena TB, Taylor CT, Rana A, Hastie ML, Gorman JJ, Hall RA. (2015) The Chikungunya Virus Capsid Protein Contains Linear B Cell Epitopes in the N- and C-Terminal Regions that are Dependent on an Intact C-Terminus for Antibody Recognition. Viruses. 8;7:2943-64.
Taylor A, Liu X, Zaid A, Goh LY, Hobson-Peters J, Hall RA, Merits A, Mahalingam S. (2017) Mutation of the N-Terminal Region of Chikungunya Virus Capsid Protein: Implications for Vaccine Design. mBio. 21;8(1).
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