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2G4 - Recognises double-stranded RNA - suitable for use in IFA, ELISA, immunoaffinity isolation.
This purchase is for an approximately 20 microgram/ml aliquot of supernatant that has not been further purified. Costs for standard shipping are included in the price.
The product data sheet can be downloaded here.
Target: long double-stranded RNA
Produced in: Mouse
Isotype: IgM, Kappa chain
Specificity: Specific for long double-stranded RNA provided that the length of the helix is >30 bp. While 2G4 can recognise 30 nt dsRNA, its reactivity is optimal for dsRNA of 50 nt or greater. The recognition is independent of the nucleotide composition. 2G4 does not bind single-stranded RNA, ssDNA, dsDNA or RNA:DNA hybrids.
Applications: For Research Use Only. IFA, ELISA, immunoaffinity isolation.
IFA: shown to be effective following fixation using 4% formaldehyde or 100% acetone.
ELISA: Can be used in a capture ELISA, or in fixed cell ELISA following 4% formaldehyde or ≥ 20% acetone. Note that for the detection of dsRNA replication intermediates for some viruses requires 80% acetone fixation or 4% formaldehyde.
Storage: Contains 0.02% sodium azide as a preservative. Store at 4 °C.
Can be aliquoted and stored frozen, but freeze/thawing must be avoided.
A safety data sheet for 0.05% sodium azide solution can be accessed here.
O'Brien CA,Hobson-Peters J,Yam AW, Colmant AM, McLean BJ, Prow NA, Watterson D, Hall-Mendelin S, Warrilow D, Ng ML, Khromykh AA, Hall RA. 2015. Viral RNA intermediates as targets for detection and discovery of novel and emerging mosquito-borne viruses. PLoS neglected tropical diseases 9:e0003629.
Blouin AG, Ross HA, Hobson-Peters J, O'Brien CA, Warren B, MacDiarmid R. A new virus discovered by immunocapture of double-stranded RNA, a rapid method for virus enrichment in metagenomic studies. Mol Ecol Resour. 2016 Sep;16(5):1255-63.
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