5.2H8 – Recognises the E2 protein of Chikungunya virus – suitable for use in IFA, ELISA, Western blot and antigen capture

This purchase is for an approximately 20 microgram/ml aliquot of supernatant that has not been further purified.  Costs for standard shipping are included in the price.

The product data sheet can be downloaded here.  For technical enquiries, please email mozzymabs@uq.edu.au

The price exclude shipping.  Shipping will be organised and charged to your shipping agent account.  We can only ship with FedEx outside of Australia; we can only ship with LabCabs, TNT or Toll within Australia.  Please email your shipping agent account number to mozzymabs@uq.edu.au or we will not be able to process your order.

Target: Chikungunya virus (CHIKV) E2 protein

Produced in: Mouse

Isotype: IgG2A

Specificity: Recognises CHIKV strains in the two major genotypes (Asian & East/Central/South African). Does not recognise the alphaviruses Ross River virus, Semliki Forest virus, Sindbis virus or Barmah Forest virus in ELISA.

Applications: For Research Use Only. IFA, ELISA, Western blot and antigen capture ELISA.

IFA: shown to be effective following fixation using 4% formaldehyde or 100% acetone.

ELISA: Can be used in fixed cell ELISA following 4% formaldehyde or ≥ 20% acetone.

Storage: Contains 0.02% sodium azide as a preservative. Store at 4 °C.

Can be aliquoted and stored frozen, but repeated freeze/thawing should be avoided.

A safety data sheet for 0.05% sodium azide solution can be accessed here.

Publications

Goh LY, Hobson-Peters J, Prow NA, Gardner J, Bielefeldt-Ohmann H, Pyke AT, Suhrbier A, Hall RA. (2013) Neutralizing monoclonal antibodies to the E2 protein of chikungunya virus protects against disease in a mouse model. Clin Immunol. 149(3):487-97.

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